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KMID : 0613820130230050629
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Journal of Life Science
2013 Volume.23 No. 5 p.629 ~ p.636
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Anti-Inflammatory Effects of Picrasma Quassioides (D.DON) BENN Leaves Extracts
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Jung Yeon-Seop
Eun Cheong-Su
Jung Young-Tae
Kim Hyun-Jeong
Yu Mi-Hee
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Abstract
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This study was performed to evaluate the anti-inflammatory and antioxidant activities of methanol extract from the leaves of Picrasma quassioides BENNET (PLME). The antioxidant effects of PLME were measured based on polyphenol and flavonoid contents. PLME was found to have 367.52μg/mg and 46.61μg/mg high polyphenol and flavonoid contents. Cell viability was determined by MTT assay. The production of nitric oxide (NO) and prostaglandin E₂ (PGE₂) was measured by Griess assay and enzyme-linked immunosorbent assay (ELISA). In order to effectively anti-inflammatory agents, we examined the inhibitory effects on the production of lipopolysaccharide (LPS)-induced NO and PGE₂ in RAW 264.7 cells. PLME significantly decreased the production of NO and PGE₂ in a dose-dependent manner, and also reduced the expression of iNOS, a COX-2 protein. In addition, PLME reduced the NF-κB, IκB phosphorylation in RAW 264.7 cells upon stimulation with LPS (100 ng/ml) for 24 h. These results provide evidence for the anti-inflammatory and antioxidant effects of Picrasma quassioides leaves.
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KEYWORD
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Anti-inflammation, Picrasma quassioides BENNET, nitric oxide, NF-κB, prostaglandin E₂
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